Plasmid and Total Protein Analyses of Extended Spectrum Beta Lactamase Producing Bacteria from Periplaneta americana (Coackroaches)
نویسندگان
چکیده
Background: The importance of plasmid-encoded Extended Spectrum Beta Lactamase (ESBL) organisms in the dissemination multi-drug resistance and complicated infections are contributory factors to treatment failure. Despite these, there is a paucity information regarding plasmid-borne associated infection from primary sources including cockroaches while studies correlating total protein ESBL also limited especially cockroaches. objective this study was therefore aimed at determining presence plasmid as well their profiles.
 Materials Methods: Bacterial isolation, characterization molecular identification were carried out our previous work following standard recommended techniques. These molecularly identified which delineated into twenty-two (22) further subjected analyses using alkaline lysis Sodium Dodecyl Sulfate Poly Acrylamide Gel Electrophoresis (SDS-PAGE) respectively.
 Results: Results obtained identifies 18(81.8%) isolates be harbouring plasmids different weights (2-21.5kbp). profile non-plasmid encoded reveals differential expression patterns except for two non- that have similar (isolates 2 3).
 Conclusion: There need monitor epidemiological distribution resistance. Improving environmental sanitation through proper disinfection carrier insects may means curtailing such spread.
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ژورنال
عنوان ژورنال: African Journal of Health Sciences
سال: 2023
ISSN: ['1022-9272', '2306-1987']
DOI: https://doi.org/10.4314/ajhs.v36i1.10